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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
Tumor Necrosis Factor α, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor <t>necrosis</t> <t>factor-α</t> ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.
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Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor necrosis factor-α ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.

Journal: iScience

Article Title: Development of immune-derived molecular markers for coronary heart disease via multimachine learning

doi: 10.1016/j.isci.2026.114853

Figure Lengend Snippet: Treprostinil promotes endothelial cell survival and viability (A) TUNEL staining showed that treprostinil significantly inhibited the apoptosis of human umbilical vein endothelial cells (HUVECs) induced by tumor necrosis factor-α ( n = 3 biological samples per group). Scale bars, 50 μm (B–D) CCK8 and qPCR analyses suggested that treprostinil promoted cell viability and reduced inflammation in HUVECs ( n = 6 biological samples per group). Two-tailed unpaired Student’s t test was used to calculate statistical significance. Data are represented as mean ± SEM. ∗ represents p < 0.05, ∗∗ represents p < 0.01, and ∗∗∗ represents p < 0.001.

Article Snippet: Tumor necrosis factor-α , MedChemExpress , HY-P1860.

Techniques: TUNEL Assay, Staining, Two Tailed Test